Key words: Listeria monocytogenes, poultry, processing plant, evisceration. ABSTRACT . Listeria antisera (Denka Seiken, Tokyo, Japan), accord- ing to the . Caracterização feno e genotípica de cepas de Listeria monocytogenes isoladas They were also serotyped (Denka Seiken, Japan), and sub–typed by PFGE. Antisera for the typing of Listeria Group O and H antigens. Aids public health and food testing laboratories in identifying sources of infection/contamination to.

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Picking seikem or a small number of colonies following an enrichment protocol for serotyping may therefore not include all serotypes present in the original sample. According to Rocourt et al.

Genomic fingerprinting of 80 strains from the W. No strain was resistant to ampicillin, gentamicin, trimethoprim and vancomycin Table 1. Nenhuma das cepas foi resistente aos antimicrobianos testados; entretanto, sete tiveram valores da CIM aumentados para o sulfametoxazol.

Academic Press, London, p.

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Thus, the Brazilian data on antibiotic resistance by L. Rabourne RM Monkey, clinical 4b 4b R.

Lindsay for facilitating sharing of strains. Finally, it is known that the cases documented here may not represent the true incidence in the community, especially sdiken regard to perinatal infection, once many laboratories of the region covered by this study do not often send the isolates for further characterization by the Regional Lab.


Characterization of brazilian Listeria monocytogenes strains using DNA macrorestriction patterns. Antigen components of each L.

Group I can be clearly divided in two clusters A and B with all the strains from cluster A belonging to serotype 4b. Services on Demand Journal. The ELISA serotyping protocol described in this work is a means to attempt to rectify the ambiguities and intra- and interlaboratory variability inherent to the traditional serotyping method, while at the same time allowing laboratories to perform more analyses with significant cost and time savings.

Filters were washed twice for 10 min each in wash buffer to remove cell debris and incubated in 20 ml of casein blocking listerja for 1 h. Serotyping of Listeria monocytogenes and related species.

Nevertheless, these data were of particular concern since emergence and dissemination of antibiotic resistance in clinical isolates of L. The analysis of macrorestriction patterns obtained by PFGE and the combination of the results obtained with both restriction enzymes, ApaI Fig.

Palumbo1 Monica K. Epidemiology of human listeriosis and seafoods. Wiedmann for listeriz L.

Incidence of Listeria monocytogenes in nature. Wesley RM Coleslaw 4b 4b I. January 11, ; Approved: After digestion with ApaI and AscI, the strains were distributed in 3 different groups according to their profile. Species and serovars of the genus Listeria isolated from different sources in Brazil from to These strains were isolated from various sources, including produce, meats and dairy products, food processing environments, animals and humans, soils, and environmental samples.


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Listeriosis has been mainly reported by those countries, with few or even no reports from Africa, Asia and South America. In this study, all the strains were susceptible to the antibiotics tested, except for sulfamethoxazole. Listeria monocytogenes ; serotypes; antibiotic resistance; genotypic characterization; listeriosis. All subsequent steps were performed at room temperature. Listeria monocytogenes is a gram-positive, food-borne bacterial pathogen that causes listeriosis in susceptible individuals 4.

Modifications to this scheme proposed by Garcia et al.

From January to May13 isolates from clinical materials seven from cerebrospinal fluid, five from blood and one from a liquid collected from an aorta prosthesis of 12 patients 11 adults and one newborn were identified as L.

Colony lifts on nitrocellulose were probed sequentially using each O-factor antiserum as the primary antibody and a unique color alkaline phosphatase substrate to correspond to each primary antibody. In addition to those strains serotyped by both methods, 97 Eeiken. Detection and differentiation of Listeria spp.