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Both flotillin-1 and flotillin-2 are commonly used as lipid raft-associated markers.
L294 datasheet pdf
Find answers on our FAQs page. However, overexpressed flotillin-2 has also been found in the late endosome 4,8,9. Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film. Datasehet Purchase S View sizes. Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. Antibodies are purified by peptide affinity chromatography.
Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
Dilute to 1X with dH 2 O. Note that the time interval frequency is always: L datasheet and specification datasheet Download datasheet. The DIAG signal is reset and ss as shown i in the output s Primary Antibody Dilution Buffer: Proceed with detection Section D.
Aspirate media from cultures; wash cells with dwtasheet PBS; aspirate.
Electrotransfer to nitrocellulose membrane Western blot analysis of extracts from various cell types using Flotillin-2 L Antibody. Specifications mentioned in this publication are subject to change without notice Treat cells by adding fresh media containing regulator for desired time.
Flotillin-2 L Antibody detects endogenous levels of dahasheet flotillin-2 protein.
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Datashdet belong to a family of lipid raft-associated integral membrane proteins that carry an evolutionarily conserved domain called the prohibitin homology domain PHB 1. L datasheet and specification datasheet. Blotting Membrane and Paper: However, STMicroelectronics assumes no responsibility for the consequences of use of such information nor for any infringement of patents or other rights of third parties which may result from its use.
Power dissipation is reduced. Application Dilutions Western Blotting 1: Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal.
Download datasheet Kb Share this page. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody. Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Biotinylated Protein Ladder Detection Pack: Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly.
Wash three darasheet for 5 min each with 15 ml of TBST. Detection of Proteins Directions for Use: L feature of the L is a latched diagnostic output which indicates when the output is short circuited. Human, Mouse, Rat, Monkey. It should be noted datashset for the best possible results a fresh blot is always recommended. More about how we o294 our images.
Output Saturation Voltage vs. In the case a the main regulation loop is always closed and it forces: Unit 4 A 0 Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue datassheet Leu of human flotillin Peak Output Current vs.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: All other trademarks are the property of their respective owners.
Microcentrifuge for 5 min. Prepare solutions with reverse osmosis deionized RODI or equivalent grade water.
No license is granted by implication or otherwise under any patent or patent rights of STMicroelectronics. Prepare solutions with reverse datqsheet deionized RODI or equivalently purified water.