Download Citation on ResearchGate | Interferencias por terapia con anticuerpos monoclonales en electroforesis e inmunofijación de proteínas séricas. electroforesis proteinas sericas pdf. Quote. Postby Just» Tue Aug 28, am. Looking for electroforesis proteinas sericas pdf. Will be grateful for any. La electroforesis de proteínas en suero mide las gammaglobulinas y otras proteínas. Esta prueba se podría pedir para diagnosticar trastornos.
|Published (Last):||9 November 2014|
|PDF File Size:||12.33 Mb|
|ePub File Size:||3.94 Mb|
|Price:||Free* [*Free Regsitration Required]|
Los caballos fueron divididos en dos grupos: Commercial fluorescein conjugated rabbit anti-horse immunoglobulin Nordic, Tilburg, Netherlands was used at a dilution of 1: Large animal internal medicine. Babesiosis is an important disease from both economic and animal health point of view, because it involves substantial losses wlectroforesis death and by decrease in animal production.
electroforesis proteinas sericas pdf
More significant clinical manifestations noted in electroforesiis Group II horses with babesiosis. Symptoms may include steady weight loss and slight anaemia usually following exercise or stress situations Hailat et al Los caballos fueron divididos en dos grupos: The plasma proteins, dysproteinemias, and immune deficiency disorders. In our study, the median obtained for the serum total proteins in the Group II was 7. This occurs mainly in horses with a more severe hypergammaglobulinemia and hepatic damage, although the immune response in some animals may produce an increase of immunoglobulin too small to be detected through routine electrophoresis techniques JainStockham and Scott Veterinary immunology an introduction.
Blood samples were obtained under non-stress conditions by jugular venipuncture using vacutainer tubes. Serum electrophoresis from healthy horses is characterized by the absence of prealbumin region and by six different bands: Although it has been reported that horses are most susceptible to infection, the disease can affect sericad, donkeys, their hybrids and wild equidae Okamura et al Med Vet 6, Finally, 12 of the sick animals displayed a ratio lower than 1 and a low albumin concentration was observed in the majority of them.
A comparison of complement fixation and immunofluorescent antibody tests in a survey of the prevalence of Babesia equi and Babesia caballi in horses in the Sultanate of Oman.
However, in the disease such as in equine babesiosis, the serum concentration of the proteins can change significantly Kumar et al Amido-Black Atom Biosystems, Spain staining was used, and a solution of methanol, acetic acid, and water Manual of equine emergencies.
Hypoalbuminemia is usually related to chronic cases and a prolonged course. Babesiosis was diagnosed by indirect immunofluorescence antibody assay. Es necesario que se conozcan los patrones del proteinograma y de la inmunofijacion de los anticuerpos monoclonales utilizados en cada hospital con la finalidad de evitar informar falsos positivos.
A cellulose acetate supporting medium for zone electrophoresis. However, until now zone-electrophoresis to evaluate changes in protein fractions has not been applied to the serum in equine babesiosis, although it is very useful from a clinical point of view because it is less time consuming.
Babesiose equine a Babesia equi: In babesiosis, the immunity mechanisms are mainly of humoral nature Morris et alalthough the protozoa can activate a cellular-type immune response Tizard Evaluation of an indirect fluorescent antibody electroforrsis to diagnose Babesia equi infection in horses. Serum proteins migrated in an electric field of volts for 65 minutes.
Análisis de globulinas: Información en MedlinePlus sobre pruebas de laboratorio
Arch Med Vet 42, Clinical biochemistry of domestic animals. More significant clinical manifestations noted in the Group II horses with babesiosis. In babesiosis, the immunity mechanisms are mainly of humoral nature Morris et alalthough the protozoa can activate a cellular-type immune response Tizard Symptoms may include steady weight loss and slight anaemia usually following exercise or stress situations Hailat et al Vet Parasitol In addition, animals with babesiosis frequently develop renal disease resulting from dehydration and haemoglobinuria by intra-vascular haemolysis produced by the parasite Navarrete y Serrano Trop Anim Health Prod 12, McCudden 15 Estimated H-index: Louis, USA, Pp Positive and negative control sera were kindly supplied by Professor G.
Commercial fluorescein conjugated rabbit anti-horse immunoglobulin Nordic, Tilburg, Netherlands was used at a dilution of 1: Identification of immunoreactive polypeptides of Babesia equi parasite during immunization. Babesiosis was diagnosed by indirect immunofluorescence antibody assay. Diseases caused by protozoa.
Serological tests were performed according with Callow et alDonnelly et al a, bSoule et aland Habela et al Genzen 1 Estimated H-index: Barta Electroflresis, SS Pourciau. Arch Med Vet 42, In order to produce clinical signs, it is considered that the serum albumin level must decrease until some values below Serum proteins have a negative charge, so they migrate in an electric field and can be separated from each other in different bands zones.
Although this technique provides very useful information about the quantitative alterations of the serum protein fractions seericas to the disease, it is not commonly used in equine medicine.
Análisis de globulinas
Serum was obtained by centrifugation for 10 minutes at g using serum-separator tubes. Each band is made up of a group of individual proteins which can be identified by this technique Barta and Pourciau The aim of the present work was characterize the serum electrophoretic pattern in horses suffering from babesiosis and to evaluate its application in the diagnosis by the study of the quantitative alterations in the serum proteins associated to this disease.
J Equine Vet Sci 26, Serum protein electrophoresis as a prognostic marker of chronic liver disease in dogs.