Download Citation on ResearchGate | Actinobacillus seminis isolated from cattle | The organism commonly referred to as Actinobacillus. Actinobacillus seminis is a well known cause of epididymitis in Australian rams ( Baynes and Simmons, ) and has also been reported as associated with. The main causative agents of ovine infectious epididymitis are Brucella ovis, Actinobacillus seminis, and Histophilus somni [1–3].

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Please review our privacy policy. These changes were observed in A method for the quantitative recovery of protein in dilute solution in avtinobacillus presence of detergents and lipids. Al-Katib and Dennis recommended continual surveillance against the introduction of infected rams into a clean flock and identifying and removing infected animals.

Actinobacillus seminis (Baynes and Simmons) Sneath and Stevens ATCC &r

In Baynes and Simmons 7 reported having isolated a microorganism from 3 rams with epididymitis. Based on clinical history, colony morphology and a sparse biochemical profile Actinobacillus seminis was suspected. A, extracts of each bacterium were exposed to antibody against A. Contagious ovine epididymitis is defined as infection of the testicle and epididymis in rams.


The absence of inflammatory reaction and bacterial isolation from only two testes of all infected animals indicate that A. The objective of our study was to identify a protein specific to A.

This conclusion was based on the reaction of hyperimmune serum of rabbits inoculated with whole cells of A. The bacterial isolate was catalase positive sekinis oxidase negative. Evers cautioned against a test and cull program based on A.

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All rams inoculated with A. A previous study has shown that rams inoculated with Catinobacillus. However, it is still necessary to conduct tests with serum from animals naturally infected with microorganisms other than A. A titer of 1: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Actinobacillus seminis – Wikipedia

Gross findings in rams seimnis infected with Actinobacillus seminis. The isolate was further investigated by API32E biochemical testing which didn’t result in a confirmed identification. Electron microscopy For negative staining, cultured A.

For scanning electron microscopy, cultured A. Bacterial subcellular fractions were obtained according to the method described by Morton et al 11 inwith some modifications. Immunoblot analysis of Actinobacillus seminis microvesicles, showing recognition of the kDa protein by antibody against A. The ewes were running in a pasture paddock in the Orange district of NSW and were supplemented with a mix of grain oats and manufactured sheep pellets.


They were fed hay and commercial ration throughout the experiment. Membrane vesicles associated with cells from A.

Ovine brucellosis caused by B. One ram had purulent exudate and diffuse hemorrhage around the tail of the left epididymis Figure 5 d.

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All reagents were from Sigma Chemical Company, St. Abstract Actinobacillus seminis is a gram-negative bacterium of the Pasteurellaceae family that is involved in ovine epididymitis. To visualize the A.

While the problem of stillborn lambs resolved, an examination of the ram flock may have also been useful. Al-Katib and Dennis, However, other isolates identified as Actinobacillus spp.